Studies on fungi in West Africa helped to expand gradually the list of WEF species of which more than 70 have been listed to date. Particularly in Benin, the total diversity of fungi is expected to be about 16842 species; All the taxonomic work undertaken reveals that only up to 2.6 % of this estimation is identified until to specific level. The rest, i.e., almost 97.4 % of the species, have not been collected and reliably identified. With the loss of natural habitat of fungi in Bassila region due to human activities (selective cutting of partner trees, deforestation, etc. it is urgent to study their diversity in order to better preserve them. Despite past conservation actions in this region, their habitats continue to be disturbed. Also, during the first two Rufford projects, we collected and documented the use of twenty-four (24) unknown WEF species. The populations have attributed therapeutic and nutritional virtues to these species. But these species and their ecology are unknown. In addition, the chemical compounds giving them their medicinal and nutritional virtues are not analysed and documented. Consequently, additional actions are needed for their long-term effective conservation.